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Optical Analytics in the Capital Region Berlin-Brandenburg

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46 Bioanalytics Applied

46 Bioanalytics Applied Laser Sensing in Complex Biosystems The ALS ComBi group at the Department of Physical Chemistry at the University of Potsdam pursues the realization of laser-based sensing techniques and their application in the life sciences. Thereby, bio-targets of different size and complexity will be characterized. The central infrastructural development was the establishment of a microscopy application lab. Here, multiplexing concepts using spatial, spectral or time-resolved separation (e.g. 1P/2P-excitation, FLIM/PLIM) and single molecule detection (e.g. FCS) provide information about spatio-temporal interactions of cellular parameters. For cell-manipulation, fluorescence microscopy can be combined with a nanofluidics-AFM (FluidFM) for delivering fL-volumes with high spatial accuracy. For studying the subcellular architecture, super-resolution microscopy techniques (easy-STED, dSTORM) with diffraction-unlimited resolution down to 40 nm are established. In addition, ALS ComBi focusses on the development of two complementary optical biosensor concepts based on SOI-technology with waveguides and photonic crystals or on optical fibers with inscribed fiber Bragg gratings. The biosensors will be adapted for label-free detection with high sensitivity and multiplexing capabilities in small sample volumes without extensive sample preparation. Non-invasive Measuring and Imaging As age-related illnesses such as cancer, cardiovascu-lar and neurodegenerative diseases become more and more relevant, new approaches and improved methods for medical diagnosis and therapy are required. Quantitative and differential-diagnostical methods help to determine early, precise and individual treatment decisions by enabling accurate and reliable identification of disease-specific parameters. Optical measurement and imaging techniques provide valuable contributions to this end. Non-invasive optical medical imaging © PTB Hierarchy of complex Biosystems ranging from nm- to cm-scales. © Uni Potsdam At the department “Biomedical Optics” of the National Metrology Institute of Germany (PTB) measurement procedures in this field are developed, examined and tested, in cooperation with clinical partners. Flow-cytometry, microscopic measurement techniques and quantitative nucleic acid diagnostics of cells and (bio-) molecules underpin metrology to support laboratory diagnostics. One goal of this work is the development of reference measurement procedures and values, to enable & support the federally mandated quality assur-ance of tests in laboratory medicine. Non-invasive quantitative determination of important biomarkers (e.g. concentration of haemoglobin, oxygen saturation) in tissue, as well as in-vivo imaging of pathological changes using fluorescencelabelled probes, round out the tasks of the department. For this purpose, opto-spectroscopic measurement and imaging techniques are developed and examined. 1 1

Bioanalytics 47 Mobile Foodstuff Analysis JTL-BioTec.Analytics develops and manufactures innovative instruments for milk analysis. Its NIR spectrometers ascertain the fat and sodium content of raw milk and check for mastiti during processing. In addition, the company also supplies automatic freezing-point osmometers to determine osmolality. Automated Sample Handling OneK+ Eye Tracker © CHRONOS VISION Ophthalmology The examination and diagnosis of eye movements and related disorders provides important information on a variety of diseases above and beyond the field of ophthalmology. The Chronos Eye Tracker (C-ETD) from CHRONOS VISION makes it possible to conduct a comprehensive examination of three-dimensional eye movement. Also, refractive surgery (e.g. Lasik procedure) demands precise real-time measurement of the position of the eye. During each laser operation, the position of the eye is measured by the Eye Tracker and relayed to the laser guidance control. Thus, the laser firing pattern compensates for any changes in eye position. Rapid tracking optimizes treatment outcome and shortens treatment time to the benefit of patients. As original equipment manufacturer, CHRONOS VISION currently supplies an OneK+ Eye Tracker with a real-time tracking rate of more than 1000 Hz together rate and an extremely short latency period. In addition, the software solution DeyeRECTOR ® provides modules that complete robust and precise eye tracking during ophthalmic surgery. Automation plays an important role in medical technology and the pharmaceutical industry. This includes, for example, pipetting or optical analysis of biological samples. The Cyto- Fa analysis system by pi4_robotics combines robot-based liquid handling with automated image acquisition of biological samples in a compact laboratory instrument. Laboratory instrument CytoFa © pi4_robotics GmbH CytoFa uses a motorized microscope with a high-resolution camera and a 3-axis robot, along with an additional axis of rotation for handling liquids. Its spatial isolation and integrated temperature control make CytoFa particularly well suited for handling light- and temperature-sensitive materials. The microscope images can be presented and stored either manually or automatically in different layers (z-stack). A variety of lighting and contrast methods can be set in the control software depending on the configuration of the microscope; these settings are then integrated into the automated process. This makes possible, for instance, fluorescence imaging of biological samples. 2 2

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