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Cluster Report Photonics in the Capital Region Berlin-Brandenburg

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72 Cluster Report Optics and Photonics – Biophotonics and Ophthalmic Optics Automation also plays an important role in medical technology or the pharmaceutical industry. This includes, for example, pipetting tasks or the optical analysis of biological samples. The CytoFa analysis system from pi4_robotics GmbH combines robot-based liquid handling with automated image acquisition of biological samples in a compact laboratory device. The CytoFa contains a mororised microscope with a high-resolution camera and a 3-axis robot with an additional rotary axis for liquid handling. Due to its spatial isolation and integrated temperature control, CytoFa is particularly suitable for the handling of light- and temperature-sensitive materials. Depending on the microscope configuration, various illumination and contrast methods can be set in the control software and integrated into the automated process. With this methods fluorescence images of biological samples can be provided, for example. RF-KombiSCAN as laboratory device © Fraunhofer IZM the UV portion in the sun’s rays. sglux is also a member of the UV For Life Consortium, which addresses the development of UV LEDs (see chapter 2.2 “Lighting Technology”). The Ferdinand-Braun-Institut, Leibniz-Institut fuer Hoechstfrequenztechnik (FBH) develops laser beam sources for Raman spectroscopy. These include diode lasers that emit light with two wavelengths at a fixed distance of about 1 nm from a chip. This makes them ideal for “Shifted Excitation Raman Difference Spectroscopy (SERDS)”. SERDS allows Raman signals to be separated from interfering backgrounds such as fluorescence or ambient light. In combination with micro-optics, the lasers come in small sizes, allowing mobile applications in medicine. FBH uses this technology, among other things, to provide beam sources in the yellow wavelength range. CytoFa laboratory device for robot-based liquid handling © pi4_robotics GmbH In 2003, sglux GmbH was founded by scientists and technicians working in the field of optical semiconductor development. They are experts in UV radiation and produce components for measuring ultraviolet (UV) radiation. In addition to waterworks, the beverage industry is one of sglux’s customers. However, the sensors are also used in dialysis machines, to monitor heating burner flames, and to measure Mobile analysis technology is also being developed at the Fraunhofer Institute for Reliability and Microintegration IZM. The RF-KombiSCAN is able to determine the quantity and composition of various substances by their different irradiation wavelengths. This new, portable, handheld optical measuring device radically simplifies and accelerates measurement procedures by integrating fluorescence and Raman spectroscopy. The mobile scanner can be used both as a laboratory research device and in industrial applications. Chemical reactions and separation operations in liquid systems are often diffusion-controlled. A reduction of the reaction systems (“lab-on-a-chip”) can therefore significantly reduce the reaction time and also the sample consumption and thus greatly simplify medical analyses. Microchips with a total size of only a few square centimetres open up the possibility of integrating several functional reaction units in a very small space. At the Leibniz-Institut für Analytische Wissenschaften – ISAS e. V., corresponding methods and structures are being researched. In combination with fast and sensitive detection methods, a fully miniaturised total analysis system (µTAS) can be set up to automate sample injection, separation, and detection.

Cluster Report Optics and Photonics – Biophotonics and Ophthalmic Optics 73 Fluorescence in vivo Imaging The principle of fluorescence in-vivo imaging is based on the properties of fluorescent dyes which are in the excited state when irradiated with certain wavelengths and emit fluorescence to return to the ground state. greateyes GmbH, a Berlin specialist for high-resolution cameras, offers a camera that is particularly sensitive in near-infrared to record these emissions. Fluorescence in vivo imaging is used, for example, in detecting the presence of cancer cells in lymph nodes. An intravenously applied dye is absorbed by the lymph node tissue. The detection of the weak fluorescence penetrating the tissue requires a highly sensitive camera and the aid of a special filter. In the course of a research project on time-resolved optical nanoscopy, a wide-field fluorescence microscope with the ultra-high spatial and time resolution is being set up to perform nanoscopic fluorescence experiments using time- and location-correlated single photon detection. The technique is designated TSCSPC (time- and space-correlated single photon counting) or FLIN (fluorescence lifetime imaging nanoscopy). For example, the fluorescence lifetime distribution of photosynthetically active proteins in unicellular organisms can be detected with unprecedented precision in order to investigate important regulatory processes in photosynthesis. Using biological or chemical reference structures, the aim is to determine to what extent the technology is suitable for breaking through the diffraction limit caused by the spatial resolution in optical microscopy. A leading manufacturer of single photon detectors is Becker & Hickl GmbH. The company, also based in Berlin, not only offers highly efficient detectors, but also picosecond lasers and analysis software as well as its own confocal fluorescence microscope. Scattered light image (left), superimposed with the fluorescence signal on the GE 1024 1024 DD NIR camera (right) © greateyes GmbH Time-resolved optical nanoscopy The Department for Bioenergetics at TU Berlin’s Institute of Chemistry applies microscopy and spectroscopy, electrophysiological methods, and their combination with optical methods to living cells. Wide-field fluorescence microscopy with high spatial and time resolution single photon detectors for multichannel FLIM measurements enable spatially resolved microscopy of dynamic processes and simultaneous fluorescence correlation spectroscopy in each pixel with 100 ps temporal resolution and a measurement duration of 10 µs. These techniques overcome previous limits in precision, parallelisation, and speed. They have a particularly high application potential in industrial projects, in pharmaceutical drug research, and cell-based diagnostics. Laser Technology in Medical Therapy For the surgeon, light can do so much more than just provide orientation. It can also be used as a tool, such as laser light which is increasingly being used for surgeries. Newport Spectra-Physics GmbH is one of the world’s largest companies in the field of laser surgery and has its German branch in Stahnsdorf, Brandenburg. The company mainly develops and produces diode-pumped femtosecond lasers. In addition to industrial applications, their applications extend to life and health sciences, microscopy, and biotechnology. The ultra-fast lasers are suitable for both LASIK surgery for eyes and other precision laser surgery.

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